Product Name

Kanamycin disulfate salt from Streptomyces kanamyceticus

General description

Kanamycin sulfate is a broad spectrum minoglycoside-antibiotic It is used as an additive in culture media for the isolation of group D Streptococci on Kanamycin Esculin Azide Agar (Mossel et al.) or for selection of transformed plant cells containing the neomycin phosphotransferase (for kanamycin resistance); on a kanamycin-medium.

Synonym

Kanamycin A, Kanamycin acid sulfate

Assay

≥670 µg/mg (Dry Basis, as Kanamycin)

CAS Number

64013-70-3

Formula

C¹⁸H³⁶N⁴O¹¹ · 2H²SO⁴

Molecular Weight

680.66

Suitability

BioReagent, suitable for cell culture, etc.

l  Physical and Chemical Information

Appearance

White to Off White Powder

Solubility(25°C)

Water

≥5mg/mL

l  Biological Information

Biochem/Physiol  Actions

Mode of Action: The product acts by binding  to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding. Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Application

Kanamycin sulfate is a broad spectrum minoglycoside-antibiotic It is used as an additive in culture media for the isolation of group D Streptococci on Kanamycin Esculin Azide Agar (Mossel et al.) or for selection of transformed plant cells containing the neomycin phosphotransferase (for kanamycin resistance); on a kanamycin-medium. Kanamycin has been used for electrophysiological recordings of outer hair cells and inner hair cells. It is used to inhibit shoot regeneration from Siberian Elmleaf explants. It is recommended for use in cell culture applications at 100 mg/mL.

l  Storage

Storage temp.

−20°C

l  Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l  References

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov