l General Information |
Product Name | Colcemid(Demecolcine) |
General description | Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase. |
Synonym | Demecolcine, N-Deacetyl-N-methylcolchicine |
Purity | ≥98%(HPLC) | CAS Number | 477-30-5 |
Formula | C21H25NO5 | Molecular Weight | 371.433 |
Suitability | BioReagent, suitable for cell culture, etc. |
l Physical and Chemical Information |
Appearance | Faint yellow to yellow and faint beige to beige and yellow-green solid |
Solubility(25°C) | Ethanol | soluble |
l Biological Information |
Biochem/Physiol
Actions | Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase. |
Application | Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis. |
l Storage |
Storage temp. | −20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
