Product Name

Melittin from honey bee venom

General description

Melittin is hydrophobic in nature except for a region with Lys-Arg-Lys-Arg sequence near C-terminal end. This structural characteristic makes melittin a highly surface-active and a powerful, direct haemolytic agent.† The encoded protein containins 26 amino acids. Monomeric form of melittin has a molecular weight of 2,840 Daltons and tetrameric form has molecular weight of approximately 12,500 Daltons.

Synonym

Melittin;Bee venom melittin

Purity

≥85%(HPLC)

CAS Number

20449-79-0

Formula

C¹³¹H²²⁹N³⁹O³¹

Molecular Weight

2846.46

Amino  Acid Sequence

Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH₂

Suitability

BioReagent, suitable for cell culture, etc.

l  Physical and Chemical Information

Appearance

White to Faint Tan or Faint Yellow Lyophilized  Powder

Solubility

Water

≥5mg/mL

l  Biological Information

Biochem/Physiol  Actions

Binds calmodulin in a Ca₂₊-dependent manner; inhibits Na₊-K₊-ATPase.Melittin acts as an anti-coagulating protein by increasing the time of blood clotting in vitro. Melittin inhibits the activity of S100 calcium-binding protein B (S100B) and plays a vital role in Epilepsy treatment.

Application

Melittin from honey bee venom has been used:

• In 3-(4, 5-dimethyl  thiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) assay to determine its cytotoxicity effect on the growth of human cell lines.

• To study the anti-microbial activity of melittin on the growth of Borrelia burgdorferi in in vitro conditions.

• As a positive control in hemolysis assay and as a cytotoxic agent against HeLa cells.

l  Storage

Storage temp.

−20°C

l  Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l  References

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov