Product Name

DNA Ligase from T4-infected Escherichia coli

Synonym

Polydeoxyribonucleotide Synthase, Polynucleotide Ligase, T4 DNA Ligase

Assay

4,000 U/mL

CAS Number

9015-85-4

Molecular Weight

mol wt 68 kDa

MDL number

MFCD00163508

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

l Biological Information

Biochem/Physiol Actions

T4 DNA Ligase forms an energy dependent phosphodiester age between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.

Application

Suitable for:

• Ligation of blunt ended or cohesive DNA fragments

• Ligation of cloning vector and restriction insert fragments

• Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids

• Couple RNA single strands by bridging oligonucleotide adapters

Unit Definition

One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37°C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov