Product Name

Pyruvate Carboxylase from bovine liver

Synonym

Pyruvate:CO² ligase (ADP-forming)

CAS Number

9014-19-1

MDL number

MFCD00132143

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

l Biological Information

Biochem/Physiol Actions

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.

Application

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes. The enzyme has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.

Unit Definition

One unit will convert 1.0 μmole of pyruvate and CO² to oxalacetate per min at pH 7.8 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov