Product Name

L-Glutamine Synthetase from Escherichia coli

Synonym

L-Glutamate:ammonia ligase (ADP-forming)

Assay

400-2,000 units/mg protein; Protein, ~5% Lowry

CAS Number

9023-70-5

MDL number

MFCD00131463

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off White lyophilized powder

Solubility(25°C)

H²O: soluble 0.95-1.05 mg/mL, clear to hazy

l Biological Information

Biochem/Physiol Actions

Degradative enzyme for glutamic acid. L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.

Application

L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

Preparation Note

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

Unit Definition

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov