Product Name

S-Acetyl-coenzyme A synthetase from baker's yeast (S. cerevisiae)

Synonym

Acetate CoA ligase (AMP forming), Acetate thiokinase

Assay

≥3 units/mg protein; Protein, 10-30% biuret

CAS Number

9012-31-1

MDL number

MFCD00081267

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol .

Application

S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO² fixation. It may also be used in gene  studies.

Preparation Note

Lyophilized powder containing stabilizers as potassium phosphate, sucrose, and reduced glutathione

Unit Definition

One unit will form 1.0 μmole of S-acetyl coenzyme A from acetate, ATP, and coenzyme A per min at pH 7.5 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov