l General Information |
Product Name | Topoisomerase I human |
General deion | Cellular topoisomerase I is present in retroviral particles and enhances viral cDNA synthesis. |
Assay | ≥2 units/μL |
Molecular Weight | mol wt 100 kDa | MDL number | MFCD03458765 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | buffered aqueous glycerol solution |
l Biological Information |
Biochem/Physiol Actions | Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices. |
Application | Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells. |
Preparation Note | Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent). |
Unit Definition | One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C. |
l Storage |
Storage temp. | −70°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
