Product Name

Protein Disulfide Isomerase from bovine liver

Synonym

PDI

Assay

>95% (SDS-PAGE), ≥100 units/mg protein, Protein, ~10% Lowry

CAS Number

37318-49-3

MDL number

MFCD00163364

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Tan lyophilized powder

l Biological Information

Biochem/Physiol Actions

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.

Application

Protein Disulfide Isomerase(PDI) is a ubiquitous, highly conserved redox chaperone enzyme from the thioredoxin superfamily. It is mainly located in the ER, where it assists in protein-folding and thiol-disulfide exchanges. It is used to study functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi.

Preparation Note

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Unit Definition

One unit cause a change in A650 of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov