Phosphoglucose Isomerase from Bacillus stearothermophilus
Product #：
S0856
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S0856-100UN
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956.8
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S0856-250UN
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1911
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l General Information |
Product Name | Phosphoglucose Isomerase from Bacillus stearothermophilus |
General deion | The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2. |
Synonym | D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase |
Assay | 300-1,000 units/mg protein; Protein, ≥60% biuret |
CAS Number | 9001-41-6 | MDL number | MFCD00131887 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator. |
Application | Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus. The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti. |
Preparation Note | lyophilized powder containing Tris buffer |
Unit Definition | One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30 °C. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
