Product Name

Phosphoglucose Isomerase from baker's yeast (S. cerevisiae)

Synonym

D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

Assay

Type III,≥400 units/mg protein (biuret)

CAS Number

9001-41-6

MDL number

MFCD00131887

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

l Biological Information

Biochem/Physiol Actions

Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator. The protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

Application

Phosphoglucose Isomerase is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. The enzyme has been used to convert fructose to glucose while evaluating fructose content in kudzu roots.

Preparation Note

Crystalline suspension in 2.6 M (NH⁴)²SO⁴ containing 0.1 mM magnesium acetate, pH 7.0

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov