Product Name

Phosphomannose Isomerase from Escherichia coli

Synonym

D-Mannose-6-phosphate ketol-isomerase, Mannose Phosphate Isomerase, PMI

Assay

recombinant, expressed in E. coli, ≥50 units/mg protein

MDL number

MFCD00131899

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

l Biological Information

Biochem/Physiol Actions

Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a  between glucose metabolism and mannosylation.

Application

PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn₊₊, Co₊₊, Fe₊₊, Mn₊₊ and Cu₊₊., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).

Preparation Note

Supplied as a suspension in 3.2 M ammonium sulfate

Unit Definition

One unit will convert 1.0 μmole of D-mannose 6-phosphate to D-fructose 6-phosphate per min at pH 7.6 at 25 °C, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov