Product Name

Triosephosphate Isomerase from rabbit muscle

Synonym

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

Assay

Type X, ≥3,500 units/mg protein; Protein, 60-90% biuret

CAS Number

9023-78-3

MDL number

MFCD00082080

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Application

Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.

Preparation Note

Sulfate-free, contains EDTA and borate buffer salts; This product is soluble (1 unit/ml) in 15 mM Tris buffer, pH 7.6, with 0.02% (w/v) BSA at 25 °C. Solutions should be prepared immediately before use

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov