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Triosephosphate Isomerase from baker's yeast (S. cerevisiae)

Product #: S0849
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Details

l General Information

Product Name

Triosephosphate Isomerase from baker's yeast (S. cerevisiae)

Synonym

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

Assay

Type I, ~10,000 units/mg protein

CAS Number

9023-78-3

MDL number

MFCD00082080

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

l Biological Information

Application

Triosephosphate isomerase has been used in a study to assess differential  of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Preparation Note

Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5; Dilute the product in 15 mM Tris buffer, pH 7.6,

containing 0.02% (w/v) BSA. When performing enzymatic assays, dilute below 1 unit/ml immediately before use.

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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