Product Name

D-Ribulose-5-phosphate 3-Epimerase from baker's yeast (S. cerevisiae)

Assay

50-100 units/mg protein (modified Warburg-Christian)

CAS Number

9024-20-8

MDL number

MFCD00130909

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

RPE is a metalloenzyme and has been shown to use the divalent Zn₂₊ ion predominantly for catalysis. Human D-ribulose-5-phosphate 3-epimerase (hRPE) has been shown to use Fe₂₊ for catalysis.

Application

D-Ribulose-5-phosphate 3-Epimerase is an enzyme that converts the reversible conversion of D-ribulose 5-phosphate into D-xylulose 5-phosphate, which is important for the cellular response against oxidative stress . D-Ribulose-5-phosphate 3-Epimerase is involved in the pentose phosphate pathway, pentose and glucuronate interconversions and carbon fixation. Product R3251 is from baker’s yeast and is provided as a lyophilized powder. It is useful in enzyme systems requiring low sulfate.

Preparation Note

Lyophilized and essentially sulfate-free; contains approx. 35% citrate buffer salts

Unit Definition

One unit will convert 1 μmole of D-ribulose 5-phosphate to D-xylulose 5-phosphate per min at pH 7.7 at 25°C when coupled with transketolase, α-glycerophosphate dehydrogenase, and triosephosphate isomerase.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov