Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
Product #:
S0842
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S0842-5UN
In-stock
514.8
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S0842-25UN
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1760.2
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Details
l General Information |
Product Name | Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus |
General deion | Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI. |
Synonym | 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC |
Assay | ≥1,000 units/mg protein (Lowry) |
CAS Number | 37288-19-0 | MDL number | MFCD00131896 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | White to Off White lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Used for the release of GPI anchored proteins from the membrane. |
Application | Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells. |
Preparation Note | The enzyme can be solubilized in 10 mM Tris-HCl, pH 7.4, containing 144 mM NaCl and 0.05% BSA. |
Unit Definition | One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation). |
l Storage |
Storage temp. | -20℃ |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |