Product Name

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis

Synonym

L-Phenylalanine ammonia-lyase, Phenylalanine Deaminase from Rhodotorula glutinis

Assay

Grade I, 0.8-2.0 units/mg protein

CAS Number

9024-28-6

MDL number

MFCD00131844

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

l Biological Information

Biochem/Physiol Actions

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis can be cleaved and inactivated by the proteases chymotrypsin, subtilisin, and trypsin.

Application

Phenylalanine ammonia-lyase has been used in a study to assess the effect of heat treatment on lignification of postharvest bamboo shoots. It has also been used in a study to investigate the effect of light on gene  and podophyllotoxin biosynthesis in Linum album cell culture.

Preparation Note

Solution in 60% glycerol, 3 mM Tris-HCl, pH 7.5, containing up to 0.5 M (NH⁴)²SO⁴

Unit Definition

One unit will deaminate 1.0 μmole of L-phenylalanine to trans-cinnamate and NH³ per min at pH 8.5 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov