Product Name

Heparinase I and III Blend from Flavobacterium heparinum

General deion

Heparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by Flavobacterium heparinum and contains specific sequences of heparin.

Assay

≥200 units/mg protein

Suitability

BioReagent

l Biological Information

Biochem/Physiol Actions

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.

Application

Heparinase I and III may be used for the study of heparin production during fermentation and specific activity of heparinise.

Preparation Note

Similar to other heparinase products, one solvent system that can be used to reconstitute this product is 20 mM Trizma HCl, pH 7.5, with 0.1 mg/mL BSA and 4 mM CaCl².

Unit Definition

One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I.One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov