Product Name

Chondroitinase ABC from Proteus vulgaris

Synonym

Chondroitin ABC Lyase

Assay

BSA free, 50-250 units/mg protein (using chondroitin sulfate C as substrate); Protein, ~10% Lowry

CAS Number

9024-13-9

MDL number

MFCD00130795

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Yellow lyophilized powder

Solubility(25°C)

0.01% bovine serum albumin aqueous (BSA) solution: soluble

l Biological Information

Biochem/Physiol Actions

Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl or (1-3)-α-L-iduronosyl ages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate. The molecular weight is found to be approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is found to be 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid and temperature optimum is 37 °C. 1 mM Zn₂₊ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.

Application

The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae.

Preparation Note

Reconstitute in a 0.01% BSA aqueous solution. Subsequent dilutions can be made into a buffer containing 50 mM Trizmaâ HCl, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Prepare solutions just prior to use.

Unit Definition

One unit will liberate 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose from chondroitin sulfate A or 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate C per min at pH 8.0 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov