Product Name

Chondroitinase ABC from Proteus vulgaris

Synonym

Chondroitin ABC Lyase

Assay

0.3-3 units/mg solid (using chondroitin sulfate C as substrate); Protein, ~35% biuret

CAS Number

9024-13-9

MDL number

MFCD00130795

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Tan or Light Orange lyophilized powder

l Biological Information

Biochem/Physiol Actions

Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) ages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn₂₊acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.

Application

Chondroitinase ABC from Sigma has been used to digest O-ed chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and  of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.

Preparation Note

Reconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared.

Unit Definition

One unit will liberate 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose from chondroitin sulfate A or 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate C per min at pH 8.0 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov