l General Information |
Product Name | Alginate Lyase |
Assay | ≥10,000 units/g solid |
CAS Number | 9024-15-1 | MDL number | MFCD03454593 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | Faint Beige to Beige to Brown Powder |
l Biological Information |
Biochem/Physiol Actions | Breaks down alginate or alginic acid to smaller molecules and reduces viscosity. The reaction may be terminated by adding NaOH. |
Application | Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture. |
Preparation Note | Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH. |
Unit Definition | One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C |
l Storage |
Storage temp. | 2-8°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
