Product Name

Fumarase from porcine heart

General deion

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Synonym

L-Malate hydrolyase, Fumarate hydratase

Assay

300-500 units/mg protein (biuret)

CAS Number

9032-88-6

MDL number

MFCD00131111

Suitability

BioReagent

l Physical and Chemical Information

Appearance

ammonium sulfate suspension

l Biological Information

Biochem/Physiol Actions

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Application

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

Preparation Note

Suspension in 3.2 M (NH⁴)²SO⁴, 0.05 M KH²PO⁴, pH 7.5, 0.014 M 2-mercaptoethanol

Unit Definition

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov