Product Name

Neuron-specific enolase from human brain

General deion

Neuron-specific enolase (NSE) is a neuronal form of the glycolytic enzyme enolase, which was first found in extracts of brain tissue.

Synonym

2-Phospho-D-glycerate hydro-lyase, Enolase, Neuron-specific from human brain, NSE, Thermolabile antigen A

Assay

≥95% (SDS-PAGE)

MDL number

MFCD00081785

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

l Biological Information

Biochem/Physiol Actions

Neuron-specific enolase (NSE) is expressed in all neuronal cell types, and its marks the acquisition of synaptic function. Following acute neuronal injury, NSE levels are increased in neuronal cell bodies. Increased levels of NSE in serum and cerebrospinal fluid have been used as markers for injury and neuronal cell death. Tumors derived from many cell types, including most neuronal and neuroendocrine tumors, express NSE. Neuron-specific enolase promotes survival of neurons and can provide neuroprotective effects by binding to neurons in a calcium-dependent manner.

Application

Neuron-specific enolase from human brain has been used in a study to assess human amniotic mesenchymal stem cells in the treatment of focal cerebral ischemia.  It has also been used in a study to investigate sinonasal teratocarcinosarcoma with rhabdoid features.

Preparation Note

Solution in 100 mM Tris-HCl, 5 mM MgSO⁴, 250 mM KCl, pH 5.0-5.2

Unit Definition

One unit causes the formation of 1.0 μmole of phospho(enol)pyruvate per minute at pH 6.8 at 25 °C

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov