Product Name

Enolase from baker's yeast (S. cerevisiae)

Synonym

2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase

Assay

≥50 units/mg protein

CAS Number

9014-08-8

MDL number

MFCD00131019

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White lyophilized powder

Solubility(25°C)

15 mM Tris HCl, pH 7.4: soluble 1.0 mg/mL, clear

l Biological Information

Biochem/Physiol Actions

Enolase is a metalloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis. Enolase from baker’s yeast is a homodimer containing two bound Mg₂₊ions. The molecular weight is 93.069 kDa.The peptide consists of 436 amino acids and contains a single cysteine residue. Two of the active site components include His191 and Arg414. The phosphorylated tyrosine residue present in yeast enolase forms a substrate for phosphorylation by tyrosine protein kinase. Apart from Mg₂₊, the enzyme can be activated by Zn₂₊, Mn₂₊, and Cd₂₊.

Application

Enolase from baker′s yeast has been used in a study to investigate the contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis. Enolase from baker′s yeast has also been used in a study to investigate the role of metal ions in catalysis by enolase. The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.

Preparation Note

Lyophilized powder containing Tris buffer salts; This enzyme is soluble in 15 mM Tris HCl, pH 7.4, (1 mg/ml), yielding a clear solution.

Unit Definition

One unit will convert 1.0 μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH 7.4 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov