Product Name

Carbonic Anhydrase I from human erythrocytes

Synonym

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase Isozyme I

Assay

100-500 W-A units/mg protein

CAS Number

9001-03-0

MDL number

MFCD00081467

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White Powder

l Biological Information

Biochem/Physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital processes such as pH and CO² homeostasis, transport of bicarbonate and CO², biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in various pathologies such as glaucoma, epilepsy and Parkinson’s disease.

Application

Carbonic anhydrase from human erythrocytes (HCA) has been used to study the molten-globule state of carbonic anhydrase (CA). Chaperone-like α-crystallin binds to this state of the enzyme and prevents its aggregation.The enzyme from sigma has been used for the analysis of thermodynamic stability of the enzyme.Furthermore, its clinical significance has been evaluated in human non-small cell lung cancer.

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov