Carbonic Anhydrase from bovine erythrocytes
Product #:
S0818
Image
SKU-Pack Size
Stock
Price($)
Quantity
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S0818-100MG
In-stock
634.4
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+
S0818-500MG
In-stock
2191.8
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Details
l General Information |
Product Name | Carbonic Anhydrase from bovine erythrocytes |
Synonym | Carbonate Dehydratase, Carbonate Hydrolyase |
Assay | ≥2,000 W-A units/mg protein |
CAS Number | 9001-03-0 | MDL number | MFCD00081467 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
l Biological Information |
Biochem/Physiol Actions | Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson’s disease. |
Application | Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA. |
Unit Definition | One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.) |
l Storage |
Storage temp. | 2-8°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |