Product Name

Carbonic Anhydrase Isozyme II from bovine erythrocytes

Synonym

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II

Assay

≥3,000 W-A units/mg protein

CAS Number

9001-03-0

MDL number

MFCD00081467

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White lyophilized powder

l Biological Information

Biochem/Physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO² homeostasis, transport of bicarbonate and CO², biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD4₊ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov