Product Name

Apotryptophanase from Escherichia coli

Assay

75-150 units/mg solid

CAS Number

9024-00-4

Molecular Weight

N/A

MDL number

MFCD00130539

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Light Yellow soluble powder

Solubility(25°C)

Water:0.90 - 1.10 mg/ml; Clear to Slightly Hazy; Colorless to Light Yellow

l Biological Information

Biochem/Physiol Actions

Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase.

Application

Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency .

Unit Definition

One unit releases one μg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov