Product Name

Citrate Synthase from porcine heart

Synonym

Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)

Assay

≥100 units/mg protein

CAS Number

9027-96-7

MDL number

MFCD00130808

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White ammonium sulfate suspension

Physical form

Suspension in 3.2 M NH⁴)²SO⁴solution, pH 7.0.

Solubility(25°C)

H²O: soluble 1.0 mg/mL, clear

l Biological Information

Biochem/Physiol Actions

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H²O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Preparation Note

Suspension in 3.2 M (NH⁴)²SO⁴ solution, pH 7.0. Dissolves in water to form a clear solution at 1 mg/mL concentration. This enzyme is soluble in water (1 mg/ml), yielding a clear solution.

Unit Definition

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov