Product Name

Aldolase from rabbit muscle

Synonym

D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase

Assay

≥8.0 units/mg protein; Protein, ≥80% biuret

CAS Number

9024-52-6

MDL number

MFCD00130453

Suitability

BioReagent

l Physical and Chemical Information

Physical form

lyophilized powder, essentially sulfate-free containing citrate buffer salts

l Biological Information

Biochem/Physiol Actions

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold .

Application

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3. The product is essentially sulfate-free and contains citrate buffer salts.

Preparation Note

Essentially sulfate-free containing citrate buffer salts

Unit Definition

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov