Product Name

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

General deion

Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.

pH optimum: ~7.9.

K_M for CO²: ~0.45 mM.

Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Synonym

3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco

Assay

0.01-0.1 unit/mg solid

CAS Number

9027-23-0

MDL number

MFCD00130908

Suitability

BioReagent

l Physical and Chemical Information

Appearance

partially purified powder

l Biological Information

Application

D-Ribulose 1,5-diphosphate carboxylase from spinach has been used in a study to assess catalytic by-product formation and ligand binding by ribulose bisphosphate carboxylases from different phylogenies.  D-Ribulose 1,5-diphosphate carboxylase from spinach has also been used in a study to determine RuBisCO activation kinetics and other rate and equilibrium constants by simultaneous multiple non-linear regression of a kinetic model.

Unit Definition

One unit will convert 1.0 μmole of D-RuDP and CO² to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov