Product Name

Apyrase from potatoes

Synonym

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

Assay

ATPase ≥60 units/mg protein, Protein, 30-60%

CAS Number

9000-95-7

MDL number

MFCD00130542

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Off White to Very Dark Yellow to Very Dark Brown lyophilized powder (partially purified)

Solubility(25°C)

H²O: soluble 10 mg/mL, slightly hazy

l Biological Information

Biochem/Physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis.Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Application

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).

Reaction: ATP → ADP+P_i → AMP+2P_i.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.

Preparation Note

This product is soluble in water at 10 mg/mL.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov