l General Information |
Product Name | Apyrase from potatoes |
Synonym | Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase |
Assay | ATPase ≥200 units/mg protein, Protein, 25-60% |
CAS Number | 9000-95-7 | MDL number | MFCD00130542 |
Suitability | BioReagent |
l Physical and Chemical Information |
Appearance | lyophilized powder |
Solubility(25°C) | H2O: soluble 1.0 mg/mL |
l Biological Information |
Biochem/Physiol Actions | Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. |
Application | At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1). Reaction: ATP → ADP+Pi → AMP+2Pi. Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase has been used in inhibition studies of platelet-aggregation. |
Preparation Note | Partially purified, lyophilized powder containing potassium succinate buffer salts. This product is soluble in water (1 mg/mL). One publication reports preparation of 125 units/mL stock solutions of this product in 10 mM HEPES, pH 7.8. |
Unit Definition | One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C. |
l Storage |
Storage temp. | -20°C |
l Precautions and Disclaimer |
This product is for R&D use only, not for drug, household, or other uses. |
l References |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
