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Apyrase from potatoes

Product #: S0797
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l General Information

Product Name

Apyrase from potatoes

Synonym

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

Assay

ATPase ≥200 units/mg protein, Protein, 30-60%

CAS Number

9000-95-7

MDL number

MFCD00130542

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Solubility(25°C)

H2O: soluble 1.0 mg/mL

l Biological Information

Biochem/Physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Application

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (10) and another with a low ratio (1).

Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase has been used in inhibition studies of platelet-aggregation .

Preparation Note

Partially purified, lyophilized powder containing potassium succinate buffer salts. This product is soluble in water (1 mg/mL). One publication has reported preparation of 1,000 units/mL stock solutions of apyrase in PBS.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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