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Home > Products > Life sciences > Enzymes and proteins > Apyrase from potatoes

Apyrase from potatoes

Product #: S0796
Image SKU-Pack Size Stock Price($) Quantity
  • S0796-200UN

    In-stock

    125.4

    - +

    S0796-500UN

    In-stock

    241

    - +

    S0796-1KU

    In-stock

    423.8

    - +

    S0796-2KU

    730.6

    - +

    S0796-5KU

    In-stock

    1606.8

    - +

Documents

Details

l General Information

Product Name

Apyrase from potatoes

Synonym

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

Assay

ATPase ≥3.0 units/mg protein, Protein, 30-60%

CAS Number

9000-95-7

MDL number

MFCD00130542

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

Physical form

Partially purified, lyophilized powder containing potassium succinate.

l Biological Information

Biochem/Physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Application

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (10) and another with a low ratio (1).

Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase has been used in inhibition studies of platelet-aggregation.

Preparation Note

This product is soluble in water (1 mg/mL).

Unit Definition

One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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