Product Name

Adenosine 5′-Triphosphatase from porcine cerebral cortex

Synonym

ATP phosphohydrolase, ATPase

Assay

≥0.3 units/mg protein, pH 7.8; Protein, 5.0-25.0% biuret

CAS Number

9000-83-3

MDL number

MFCD00135671

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na₊^,K₊-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA).

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na₊,K₊-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na₊/K₊-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells .

Application

ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity .

Preparation Note

Lyophilized powder containing 90% sucrose, 0.4% EDTA Na₄ and 0.06% NaCl

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na₊, K₊, and Mg₂₊.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov