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Pyrophosphatase, Inorganic from Escherichia coli

Product #: S0793
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Details

l General Information

Product Name

Pyrophosphatase, Inorganic from Escherichia coli

Synonym

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

Assay

recombinant, expressed in E. coli, ≥90%, ≥800 units/mg protein

CAS Number

9024-82-2

MDL number

MFCD00131379

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White Lyophilized powder

l Biological Information

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.

It plays an important role in protein, RNA, and DNA synthesis.

Preparation Note

Lyophilized powder in Tris-buffered salts containing protease inhibitors; Reconstitute the lyophilized enzyme in 2 ml of deionized water to obtain a 0.5 mg/ml solution in 12.5 mM Tris buffer and protease inhibitors.

Unit Definition

One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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