Product Name

Pyrophosphatase, Inorganic from baker's yeast (S. cerevisiae)

General deion

Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)

Extinction Coefficient: E_1% = 14.5 (280 nm)

pI: 4.75

This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg₂₊ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg₂₊, both ADP and ATP can be hydrolyzed if zinc is present.

Synonym

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

Assay

≥500 units/mg protein (E_1%/280)

CAS Number

9024-82-2

MDL number

MFCD00131379

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off-White powder

l Biological Information

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H²O → 2P_i.

It plays an important role in protein, RNA, and DNA synthesis.

Pyrophosphatase has been used:

as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.

as a component of PCR mixture

Pyrophosphatase has been used:

• as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.

• as a component of PCR mixture.

Preparation Note

Lyophilized powder containing 85% buffer salts; The lyophilized powder contains approximately 85% buffer salts (Tris, citric acid and magnesium chloride). This product is soluble in deionized water (2 mg/ml), yielding a clear and colorless solution.

Unit Definition

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov