Product Name

Adenosine Deaminase from bovine spleen

Synonym

Adenosine aminohydrolase

Assay

Type IX, 150-200 units/mg protein

CAS Number

9026-93-1

MDL number

MFCD00081287

Molecular weight

32.5-33 kDa

pI

4.85

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Suspension in 3.2 M (NH⁴)²SO⁴, 0.01 M potassium phosphate, pH 6.0

l Biological Information

Biochem/Physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Application

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Preparation Note

Suspension in 3.2 (NH⁴)²SO⁴,, 0.01 M potassium phosphate, pH 6.0; The enzyme preparation may be diluted in water or

buffer to a protein concentration of 1 mg/ml, producing a clear, colorless solution.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov