Product Name

Adenosine Deaminase (ADA) from calf intestine

General deion

Adenosine aminohydrolase; Adenosine deaminase (ADA) is a zinc metalloenzyme.

Assay

from calf intestine, pkg of 2 mL (10 mg); ~200 units/mg protein (At 25 °C with adenosine as the substrate.)

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6.

l Biological Information

Biochem/Physiol Actions

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Application

Deamination of adenosine analogs to the corresponding inosine analogs. Adenosine deaminase has been used to pre-treat primary Kupffer cells.

Preparation Note

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6; ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov