Product Name

Creatinase from Pseudomonas sp.

Synonym

Creatine Amidinohydrolase

Assay

recombinant, expressed in E. coli, 10-15 units/mg protein; Protein, ~70% biuret

CAS Number

37340-58-2

MDL number

MFCD00130875

Suitability

BioReagent

l Physical and Chemical Information

Appearance

lyophilized powder

l Biological Information

Biochem/Physiol Actions

Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H²O, to form urea and sarcosine.

Application

Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.

Unit Definition

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov