Product Name

Peptidyl Arginine Deiminase from rabbit skeletal muscle

General deion

Peptidyl arginine deiminase is the enzyme that converts arginine into citrulline.

Synonym

Protein arginine iminohydrolase

Assay

≥200 units/mg protein (Bradford)

CAS Number

75536-80-0

MDL number

MFCD00165226

Suitability

BioReagent

l Physical and Chemical Information

Appearance

Colorless buffered aqueous glycerol solution

Physical form

Solution in 20 mM Tris-HCl, pH 7.4, containing 10 mM 2-mercaptoethanol, 1 mM EDTA and 10% glycerol

l Biological Information

Biochem/Physiol Actions

Calcium is required for peptidylarginine deiminase activity in vitro.

Application

Peptidyl arginine deiminase has been used in a study that assessed promising novel biomarkers for the early diagnosis of rheumatoid arthritis. It has also been used in a study to investigate the autopathogenic correlation of periodontitis and rheumatoid arthritis.

Preparation Note

Solution in 20 mM Tris-HCl, pH 7.4, containing 10 mM 2-mercaptoethanol, 1 mM EDTA and 10% glycerol

Unit Definition

One unit will produce 1 μmole of N-α-benzoylcitrulline ethyl ester from BAEE per hr at 55 °C at pH 7.2.

l Storage

Storage temp.

−70°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov