Product Name

β-Lactamase from Enterobacter cloacae

Synonym

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

Assay

Type IV, 0.2-0.6 units/mg protein (using benzylpenicillin); Protein, ~60%

CAS Number

9073-60-3

MDL number

MFCD00131819

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Yellow-Tan lyophilized powder

Physical form

Lyophilized powder containing sodium phosphate buffer salts

Solubility(25°C)

0.90 - 1.10 mg/ml water; Colorless to Light Yellow; Clear to Hazy

l Biological Information

Biochem/Physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. The product is produced from Enterobacter cloacae and is provided as a lyophilized powder.

Preparation Note

Lyophilized powder containing sodium phosphate buffer salts

Unit Definition

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov