Product Name

β-Lactamase from Enterobacter cloacae

Synonym

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

Assay

Type III, 6-18 units/mg protein (using benzylpenicillin); Protein, ~10%

CAS Number

9073-60-3

MDL number

MFCD00131819

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Faint Yellow lyophilized powder

Solubility(25°C)

0.90 - 1.10 mg/ml water; Colorless; Clear

l Biological Information

Biochem/Physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. The product is produced from Enterobacter cloacae.

Preparation Note

Lyophilized powder containing sodium phosphate and sodium citrate buffer salts.

Unit Definition

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov