Product Name

Creatininase from microorganisms

Synonym

Creatinine Amidohydrolase

Assay

100-300 units/mg protein; Protein, 65-85%

CAS Number

9025-13-2

Molecular Weight

mol wt ~175 kDa

MDL number

MFCD00130877

Suitability

BioReagent

l Physical and Chemical Information

Physical form

Lyophilized powder containing sucrose and BSA as stabilizers

l Biological Information

Biochem/Physiol Actions

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

Application

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

Preparation Note

Lyophilized powder containing sucrose and BSA as stabilizers

Unit Definition

One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25 °C

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov