Product Name

PNGase F from Elizabethkingia meningoseptica

Synonym

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, Peptide N-glycosidase

Assay

recombinant, expressed in E. coli, set of 100 units nanomolar unit; ≥10 units/mg protein

CAS Number

83534-39-8

MDL number

MFCD00131222

Suitability

BioReagent

l Biological Information

Biochem/Physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-ed core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-ed asparagine as the central residue is the minimal substrate for PNGase F.

Application

Used to deglycosylate protein. PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins.

Unit Definition

One unit will catalyze the release of N-ed oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov