Product Name

PNGase F from Elizabethkingia meningoseptica

Synonym

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, Peptide N-glycosidase

Assay

≥95% (SDS-PAGE), for proteomics

CAS Number

83534-39-8

Molecular Weight

mol wt ~36 kDa

MDL number

MFCD00131222

Suitability

BioReagent

l Physical and Chemical Information

Appearance

White to Off White Powder

l Biological Information

Biochem/Physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Application

Used to deglycosylate protein. Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-ed deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Preparation Note

Before opening the vial containing PNGase F, centrifuge the vial briefly to ensure the lyophilized material is at the bottom of the tube. Prepare a 500 units/ml solution by adding 100 ml of high purity water to a vial of 50 units. This results in an enzyme solution containing ~5 mM potassium phosphate buffer, pH 7.5. Depending on the application, solutions containing higher or lower enzyme activity may be prepared with the appropriate volume of water.

Unit Definition

One unit will catalyze the release of N-ed oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov