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Home > Products > Life sciences > Enzymes and proteins > PNGase F from Elizabethkingia miricola

PNGase F from Elizabethkingia miricola

Product #: S0771
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Details

l General Information

Product Name

PNGase F from Elizabethkingia miricola

Synonym

N-Glycosidase F, Peptide N-glycosidase

Assay

≥5000 units/ml

CAS Number

83534-39-8

MDL number

MFCD00131222

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

l Biological Information

Biochem/Physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Application

Used to deglycosylate protein

Preparation Note

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Unit Definition

One unit will catalyze the release of N-ed oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

l Storage

Storage temp.

2-8°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov

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