Product Name

PNGase F from Elizabethkingia meningoseptica

Synonym

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Elizabethkingia meningoseptica, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

Assay

recombinant, expressed in E. coli, ≥5 µg protein/ml; ≥1000 U/mg protein

CAS Number

83534-39-8

Molecular Weight

mol wt ~36 kDa

MDL number

MFCD00131222

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous solution

l Biological Information

Biochem/Physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Application

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. Used to deglycosylate N-ed glycoprotein. Used to deglycosylate protein.

Preparation Note

Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.

Unit Definition

One unit will catalyze the release of N-ed oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov