Product Name

Glycopeptidase A from almonds

General deion

Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine ages.

Synonym

N-ed-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase, N-Glycosidase A, PNGase A

Assay

≥0.05 unit/mL

CAS Number

83534-39-8

MDL number

MFCD00131222

Suitability

BioReagent

l Physical and Chemical Information

Appearance

buffered aqueous glycerol solution

l Biological Information

Biochem/Physiol Actions

Hydrolyzes an N₄-(acetyl-β-D-glycosaminyl)asparagine in which the N-acetyl-D-glucosamine residue may be further glycosylated, yielding a (substituted) N-acetyl-β-D-glucoaminylamine and the peptide containing an aspartic residue.

Application

Glycopeptidase A from almonds is used for deglycosylation.It catalyzes the removal of N-ed oligosaccharide chains and converts Asn residue to Asp.

Preparation Note

Solution in 50% glycerol containing 50 mM citrate-phosphate buffer, pH 5.0, and BSA.

Unit Definition

One unit will hydrolyze 1.0 μmole of ovalbumin glycopeptide per min at pH 5.0 at 37°C.

l Storage

Storage temp.

-20°C

l Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses.

l References

1. http://www.drugbank.ca

2. https://ncit.nci.nih.gov

3. https://www.ncbi.nlm.nih.gov